THE SEATH LAB
Publications
30 / Mesoscale proximity labeling to study macro changes to chromatin occupancy
C. J. Douglas, P. Samowitz, F. Tong, A. Long, C. M. Bradley, L. Radnai, D. W. C. MacMillan, C. A. Miller, G. Rumbaugh, C. P. Seath
bioRxiv 2025.03.13.643041; doi: https://doi.org/10.1101/2025.03.13.643041​
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29 / Multi-probe photoproximity labeling of the EGFR interactome in glioblastoma using red-light
F. Tong, W. Zhou, M. Janiszewska, C. P. Seath
J. Am Chem. Soc. 2025, Accepted​
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In this manuscript we develop a carbene based probe for red-light photoproximity labeling by utilizing a sulfonium stabilized diazo group. We then exploit this method to study the interactome of EGFR through different resections obtained from a heterogeneous GBM tumor and show that EGFR has a unique interactome in brain infiltrates in GBM when compared to non-brain cancers and even the core of the same brain tumor.
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28 / Native top-down proteomics enables discovery in endocrine resistant breast cancer
F. P. Gomes, K. R. Durbin, K. Schauer, J. C. Nwachukwu, R. J. Russo, J. W. Njeri, C. P. Seath, A. J. Saviola, D. B. McClatchy, J. K. Diedrich, P. T. Garrett, A. B. Papa, I. Ciolacu, N. L. Kelleher, K. W. Nettles, and J. R. Yates III
Nat. Chem. Bio. 2025, https://doi.org/10.1038/s41589-025-01866-8​
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27 / Targeting Recycling Endosomes to Potentiate mRNA Lipid Nanoparticles
J. Shin, C. J. Douglas, S. Zhang, C. P. Seath, H. Bao*
Nano Lett. 2024, https://doi.org/10.1021/acs.nanolett.3c04415
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In this collaboration with the Bao lab we identified the protein target of the small molecule ES5 using CETSA. This represents proof of concept that targeting ANXA6 with small molecules can greatly enhance mRNA delivery - a significant barrier to the development of RNA based therapeutics.
26 / Creative approaches using proximity labeling to gain new biological insights
R. R. Milione​, B. Schell, C. J. Douglas, C. P. Seath Trends in Biochemical Sciences 2023, https://doi.org/10.1016/j.tibs.2023.12.005
25 / μMap photoproximity labeling enables small molecule binding site mapping
S. W. Huth​, J. V. Oakley, C. P. Seath, J. B. Geri, A. D. Trowbridge, D. L. Parker Jr., F. P. Rodriguez-Rivera, A. G. Schwaid, C. Ramil, K. Ah Ryu, C. H. White, O. O. Fadeyi, R. C. Oslund and D. W. C. MacMillan* J. Am. Chem. Soc 2023, https://doi.org/10.1021/jacs.3c03325
24 / Proximity labelling to study chromatin interactomes
C. P. Seath Nature Reviews Cancer 2023, 10.1038/s41568-023-00596-0
23 / Tracking chromatin state changes using nanoscale photo-proximity labelling
22 / Photoproximity Labeling of Sialylated Glycoproteins (GlycoMap) Reveals Sialylation-Dependent Regulation of Ion Transport
21 / Radius measurement via super-resolution microscopy enables the development of a variable radii proximity labeling platform
Oakley, J. V.; Buksh, B. F.; Fernández, D. F.; Oblinsky, D. G.; Seath, C. P.; Geri, J. B.; Scholes, G. D.; MacMillan, D. W. C. Proc. Natl. Acad. Sci. 2022, 119, e2203027119
20 / μMap-Red: Proximity Labeling by Red Light Photocatalysis.
Buksh B. F.; Knutson, S. D.; Oakley, J. V.; Bissonnette, N. B.; Oblinsky, D. G.; Schwoerer, M. P.; Seath, C. P.; Geri, J. G.; Rodriguez-Rivera, F. P.; Parker. D. L.; Scholes, G. D.; Ploss, A.; MacMillan. D. W. C. J. Am. Chem. Soc. 2022, 144, 6154
19 / Tracking chromatin state changes using µMap photo-proximity labeling.
Seath, C. P.*; Burton, A. J.*; MacMillan, D. W. C.; Muir, T. W. BioRxiv. https://doi.org/10.1101/2021.09.28.462236
18 / Small molecule photocatalysis enables drug target identification via energy transfer
Trowbridge, A. D.*; Seath, C. P.*; Rodriguez-Rivera, F. P.*; Li, B. X.; Dul, B. E.; Schwaid, A. G.; Geri, J. B.; Oakley, J. V.; Fadeyi, O. O.; Oslund, R. C.; Ryu, K. A.; White, C.; Reyes-Robles, T.; Tawa, P.; Parker Jr., D. L.; MacMillan D. W. C. PNAS, 2022, Accepted. https://doi.org/10.1101/2021.08.02.454797
17 / Metallaphotoredox: The Merger of Photoredox and Transition Metal Catalysis.
Chan, A.; Perry, I.; Bissonnette, N.; Buksh, B. F.; Edwards, G.; Frye, L.; Garry, O.; Lavagnino, M.; Li, B. X.; Liang, Y.; Mao, E.; Millet, A.; Oakley, J. V.; Reed, N.; Sakai, H.; Seath, C. P.; MacMillan, D. W. C. Chem. Rev. Accepted. https://doi.org/10.1021/acs.chemrev.1c00383
16 / Reactive intermediates for interactome mapping.
Seath, C. P.; Trowbridge, A. D.; Muir, T. W.; MacMillan, D. W. C. Chem. Soc. Rev. 2021, 50, 2911–2926.
15 / Selective C-F Functionalization of Unactivated Trifluoromethylarenes.
Vogt, D. B.; Seath, C. P.; Wang, H; Jui, N. T. J. Am. Chem. Soc. 2019 141, 13203-13211.
14 / Photocatalytic Approaches Towards Radical Pyridine Alkylation
Seath, C. P.; Jui, N. T. Synlett (SynPACT), 2019, 30, A-H.
13 / A Catalytic Strategy for Regioselective Arylethylamine Synthesis.
Boyington, A. J.; Seath, C. P.; Zearfoss, A. M.; Xu, Z.; Jui, N. T. J. Am. Chem. Soc. 2019, 141, 4147-4153.
12 / Radical Hydroarylation of Functionalized Olefins and Mechanistic Investigation of Photocatalytic Pyridyl Radical Reactions.
Seath, C. P.; Vogt, D. B.; Xu, Z.; Boyington, A. J.; Jui, N. T. J. Am. Chem. Soc. 2018, 140, 15525–15534.
11 / Interrogating Pd(II) Anion Metathesis Using a Bifunctional Chemical Probe: A Transmetalation Switch.
Molloy, J. J.; Seath, C. P.; West, M. J.; McLaughlin, C.; Fazakerley, N. J.; Kennedy, A. J.; Nelson, D. J.; Watson, A. J. B. J. Am. Chem. Soc. 2018, 140, 126−130.
10 / A One-pot Tandem Chemoselective Allylation/Cross-coupling via Temperature Control of a Multi-nucleophile/electrophile System.
Xu, C.; Fyfe, J. W. B; Seath, C. P.; Bennett, S. H.;† Watson, A. J. B. Chem. Commun. 2017, 53, 9139–9142.
9/ Chemoselective Sequential Control of CuAAC Ligations Using Aromatic Ynamine Protecting Groups.
Hatit, M. Z. C.; Seath, C. P.; Watson, A. J. B.; Burley, G. A. J. Org. Chem. 2017, 82, 5461–5468.
8 / Determining the Origin of Rate-Independent Chemoselectivity in CuAAC Reactions: An Alkyne-Specific Shift in Rate-Determining Step.
Seath, C. P.; Burley, G. A.; Watson, A. J. B. Angew. Chem. Int. Ed. 2017, 56, 3314–3318.
7 / Synthesis of Oxindoles and Benzofuranones via Oxidation of 2-Heterocyclic BMIDA.
Seath C. P.; Fyfe, J. W. B.; Molloy, J. J.; Watson, A. J. B. Synthesis 2017, 49, 891–898.
6 / Chemoselective Sequential Click Ligations Directed by Enhanced Reactivity of an Aromatic Ynamine.
Hatit, M. Z. C.; Sadler, J. C.;McLean, L. A.; Whitehurst, B. C.; Seath, C. P.; Humphreys, L. D.; Young, R. J.; Watson, A. J. B.; Burley, G. A. Org. Lett. 2016, 18, 1694–1697.
5 / Synthesis of 2-BMIDA 6,5-Bicyclic Heterocycles by Cu(I)/Pd(0)/Cu(II) Cascade Catalysis of 2-Iodoanilines/phenols.
Seath, C. P.; Wilson, K. L.; Campbell, A.; Mowat, J. M.; Watson, A. J. B. Chem. Commun. 2016, 52, 8703–8706.
4 / Tandem Chemoselective Suzuki-Miyaura Cross-coupling Enabled by Nucleophile Speciation Control.
Seath, C. P.; Fyfe, J. W. B.; Molloy, J. J.; Watson, A. J. B. Angew. Chem. Int. Ed. 2015, 54, 9976–9979.
3 / Speciation Control During Suzuki-Miyaura Cross-Coupling of Haloaryl and Haloalkenyl MIDA Boronic Esters.
Fyfe, J. W. B.; Valverde, E.; Seath, C. P.; Redmond, J. M.; Anderson, N; Watson, A. J. B. Chem.-Eur. J. 2015, 24, 8951–8964.
2 / A Modular Synthesis of Functionalized Phenols Enabled by Controlled Boron Speciation.
Molloy, J. J.; Law, R. P.; Fyfe, J. W. B.; Seath, C. P.; Hirst, D. J.; Watson, A. J. B. Org. Biomol. Chem. 2015, 13, 3093–3102.
1 / Chemoselective Boronic Ester Synthesis by Controlled Speciation.
Fyfe, J. W. B.; Seath, C. P.; Watson, A. J. B. Angew. Chem. Int. Ed. 2014, 53, 12077–12080.